Neuron culture from mouse superior cervical ganglion

M Jackson, W Tourtellotte - Bio-protocol, 2014 - bio-protocol.org
The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of
neurons for studying the mechanisms of sympathetic nervous system (SNS) development
and growth in vitro. The sympathetic nervous system (SNS) of early postnatal animals
undergoes a great deal of remodeling and development; thus, neurons taken from mice at
this age are primed to re-grow and establish synaptic connections after in situ removal. The
stereotypic location and size of the SCG make it ideal for rapid isolation and dissociation …