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URL: http://scholar.google.com/scholar_lookup?title=Analysis+of+relative+gene+expression+data+using+real-time+quantitative+PCR+and+the+2%28%E2%80%93%CE%94%CE%94C%28T%29%29+Method&year=2001&author=Livak&journal=Methods&volume=25&issue=4&pages=402-408&doi=10.1006%2Fmeth.2001.1262&pmid=11846609