Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system
M Ghorbal, M Gorman, CR Macpherson… - Nature …, 2014 - nature.com
Nature biotechnology, 2014•nature.com
Genome manipulation in the malaria parasite Plasmodium falciparum remains largely
intractable and improved genomic tools are needed to further understand pathogenesis and
drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by
disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions
with high efficiency. Additionally, an artemisinin-resistant strain was generated by
introducing a previously implicated polymorphism, thus illustrating the value of efficient …
intractable and improved genomic tools are needed to further understand pathogenesis and
drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by
disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions
with high efficiency. Additionally, an artemisinin-resistant strain was generated by
introducing a previously implicated polymorphism, thus illustrating the value of efficient …
Abstract
Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.
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