Hepatic differentiation from human embryonic stem cells using stromal cells
YD Yu, KH Kim, SG Lee, SY Choi, YC Kim… - Journal of Surgical …, 2011 - Elsevier
YD Yu, KH Kim, SG Lee, SY Choi, YC Kim, KS Byun, IH Cha, KY Park, CH Cho, DH Choi
Journal of Surgical Research, 2011•ElsevierOBJECTIVE: The derivation of hepatocytes from human embryonic stem (hES) cells is of
value both in the study of early human liver organogenesis and in the creation of an
unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the
generation of hepatocyte-like cells derived from hES cells. METHODS: Hepatic endoderm
cells were generated by adding activin A for 5 d-to 1-d-old embryoid bodies formed from
hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 …
value both in the study of early human liver organogenesis and in the creation of an
unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the
generation of hepatocyte-like cells derived from hES cells. METHODS: Hepatic endoderm
cells were generated by adding activin A for 5 d-to 1-d-old embryoid bodies formed from
hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 …
OBJECTIVE
The derivation of hepatocytes from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report the generation of hepatocyte-like cells derived from hES cells.
METHODS
Hepatic endoderm cells were generated by adding activin A for 5 d- to 1-d-old embryoid bodies formed from hES cells. The hepatic endoderm cells were cocultured with mitomycin treated 3T3-J2 feeder cells.
RESULTS
After co-culture with mitomycin treated 3T3-J2 feeder cells, these hepatic endodermal cells yielded hepatocyte-like cell colonies, which possessed the proliferation potential to be cultured for an extended period of more than 30 d. With extensive expansion, they co-expressed the hepatic marker AFP and albumin, indicating that they were hepatocyte-like cells.
CONCLUSIONS
We report the generation of proliferative hepatocyte-like cells from hES cells. These hES cell derived hepatic cells can effectively be used as in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.
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