[HTML][HTML] Mammalian homologues of the Drosophila slit protein are ligands of the heparan sulfate proteoglycan glypican-1 in brain
Using an affinity matrix in which a recombinant glypican-Fc fusion protein expressed in 293
cells was coupled to protein A-Sepharose, we have isolated from rat brain at least two
proteins that were detected by SDS-polyacrylamide gel electrophoresis as a single 200-kDa
silver-stained band, from which 16 partial peptide sequences were obtained by nano-
electrospray tandem mass spectrometry. Mouse expressed sequence tags containing two of
these peptides were employed for oligonucleotide design and synthesis of probes by …
cells was coupled to protein A-Sepharose, we have isolated from rat brain at least two
proteins that were detected by SDS-polyacrylamide gel electrophoresis as a single 200-kDa
silver-stained band, from which 16 partial peptide sequences were obtained by nano-
electrospray tandem mass spectrometry. Mouse expressed sequence tags containing two of
these peptides were employed for oligonucleotide design and synthesis of probes by …
