Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression
M Mandapathil, S Lang, E Gorelik… - Journal of immunological …, 2009 - Elsevier
Human regulatory T cells (Treg) have been variously defined as CD4+ CD25+, CD4+
CD25high or CD4+ CD25highFOXP3+ cells which are responsible for maintaining
peripheral tolerance. Their isolation from human peripheral blood or tissues depends on the
expression level of CD25 (IL-2Rα)—a surface marker which is also expressed on activated
effector helper T cells. CD39, a cell surface associated ectonucleotidase, can be used to
purify Treg with strong suppressor functions. The CD4+ CD39+ T cells catalyze cleavage of …
CD25high or CD4+ CD25highFOXP3+ cells which are responsible for maintaining
peripheral tolerance. Their isolation from human peripheral blood or tissues depends on the
expression level of CD25 (IL-2Rα)—a surface marker which is also expressed on activated
effector helper T cells. CD39, a cell surface associated ectonucleotidase, can be used to
purify Treg with strong suppressor functions. The CD4+ CD39+ T cells catalyze cleavage of …
