Hypomethylation of HeLa cell DNA and the absence of 5-methylcytosine in SV40 and adenovirus (type 2) DNA: analysis by HPLC
ES Diala, RM Hoffman - Biochemical and biophysical research …, 1982 - Elsevier
ES Diala, RM Hoffman
Biochemical and biophysical research communications, 1982•ElsevierMethylation of the purified virion DNA of both SV40 and adenovirus (type 2) was measured
by high performance liquid chromatography (HPLC) and compared to their hosts, African
green monkey kidney cells and Hela cells, respectively. Essentially, no 5-methylcytosine
was detected in either viral DNA. Implications for viral gene regulation by methylation are
discussed. In comparison with normal human cell DNA methylation levels, HeLa cell DNA
methylation is reduced significantly.
by high performance liquid chromatography (HPLC) and compared to their hosts, African
green monkey kidney cells and Hela cells, respectively. Essentially, no 5-methylcytosine
was detected in either viral DNA. Implications for viral gene regulation by methylation are
discussed. In comparison with normal human cell DNA methylation levels, HeLa cell DNA
methylation is reduced significantly.
Abstract
Methylation of the purified virion DNA of both SV40 and adenovirus (type 2) was measured by high performance liquid chromatography (HPLC) and compared to their hosts, African green monkey kidney cells and Hela cells, respectively. Essentially, no 5-methylcytosine was detected in either viral DNA. Implications for viral gene regulation by methylation are discussed. In comparison with normal human cell DNA methylation levels, HeLa cell DNA methylation is reduced significantly.
Elsevier
