[HTML][HTML] Stable expression of angiopoietin-1 and other markers by cultured pericytes: phenotypic similarities to a subpopulation of cells in maturing vessels during later …
C Sundberg, M Kowanetz, LF Brown, M Detmar… - Laboratory …, 2002 - Elsevier
Pericytes have been difficult cells to study because they do not maintain their characteristic
phenotype in vitro, and they begin to express fibroblast markers after only a few days in
culture. We now report methods for the isolation, purification, culture, and repurification of
human dermal pericytes from mixed cell populations using an immunoaffinity-magnetic bead
approach coupled with the 3G5 IgM monoclonal antibody that is specific for a pericyte
surface ganglioside. These purified cells could be expanded in culture, and they maintained …
phenotype in vitro, and they begin to express fibroblast markers after only a few days in
culture. We now report methods for the isolation, purification, culture, and repurification of
human dermal pericytes from mixed cell populations using an immunoaffinity-magnetic bead
approach coupled with the 3G5 IgM monoclonal antibody that is specific for a pericyte
surface ganglioside. These purified cells could be expanded in culture, and they maintained …
