We have developed a three-colour flow cytometric method to assay the contributions of
cytotoxicity and phagocytosis to antibody-dependent cell-mediated tumour cell killing. In this
assay, tumour target cells are pre-labelled with CFSE, and mixed with effector cells and a
tumour antigen-specific monoclonal antibody. After incubation of the cells with the antibody,
effector cells are labelled with PE and dead cells with PI. Using flow cytometry, dead and
phagocytosed tumour cells can be quickly and easily counted and the numbers summed to …