Mast cell heterogeneity in the gastrointestinal tract: variable expression of mouse mast cell protease-1 (mMCP-1) in intraepithelial mucosal mast cells in nematode …
CL Scudamore, L McMillan, EM Thornton… - The American journal …, 1997 - ncbi.nlm.nih.gov
CL Scudamore, L McMillan, EM Thornton, SH Wright, GF Newlands, HR Miller
The American journal of pathology, 1997•ncbi.nlm.nih.govSoluble granule chymases in rodent intestinal mucosal mast cells (IMMCs) may play an
important role in altering epithelial permeability during immediate hypersensitivity reactions.
Using a monoclonal antibody against the chymase mouse mast cell protease-1 (mMCP-1),
we have shown that it is constitutively expressed in< or= 20% of esterase-positive
(esterase+) IMMCs but not in esterase+ gastric mucosal mast cells (GMMCs) in normal
BALB/c mice. Intestinal infection with mouse-or rat-adapted strains of Nippostrongylus …
important role in altering epithelial permeability during immediate hypersensitivity reactions.
Using a monoclonal antibody against the chymase mouse mast cell protease-1 (mMCP-1),
we have shown that it is constitutively expressed in< or= 20% of esterase-positive
(esterase+) IMMCs but not in esterase+ gastric mucosal mast cells (GMMCs) in normal
BALB/c mice. Intestinal infection with mouse-or rat-adapted strains of Nippostrongylus …
Abstract
Soluble granule chymases in rodent intestinal mucosal mast cells (IMMCs) may play an important role in altering epithelial permeability during immediate hypersensitivity reactions. Using a monoclonal antibody against the chymase mouse mast cell protease-1 (mMCP-1), we have shown that it is constitutively expressed in< or= 20% of esterase-positive (esterase+) IMMCs but not in esterase+ gastric mucosal mast cells (GMMCs) in normal BALB/c mice. Intestinal infection with mouse-or rat-adapted strains of Nippostrongylus brasiliensis resulted in IMMC hyperplasia with 100% of esterase+ IMMCs expressing mMCP-1. In contrast, there was a variable response in terms of numbers of GMMCs and of the proportion expressing mMCP-1. Esterase+ mast cells in the gastric submucosa, muscularis, ear pinna, lung parenchyma, major airway submucosa, and peritoneal cavity did not express mMCP-1. The few airway esterase+ mast cells expressing mMCP-1 were, like the great majority of IMMCs and GMMCs, located intraepithelially. In conclusion, mMCP-1 is predominantly expressed by intraepithelial mucosal mast cells but not in all sites; the immunological stimulus associated with intestinal nematodiasis substantially up-regulates mMCP-1 expression by mast cells in the jejunum but not in the stomach; IMMCs and GMMCs in BALB/c mice are phenotypically and possibly functionally distinct.
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