Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanes.
JD Morrow, TA Minton, CR Mukundan… - Journal of Biological …, 1994 - ASBMB
We recently reported the discovery that a series of novel prostaglandin (PG) F2-like
compounds (F2-isoprostanes) are produced in vivo independent of the cyclooxygenase as
products of free radical-catalyzed lipid peroxidation. F2-isoprostanes are initially formed in
situ from arachidonic acid esterified to phospholipids and then released preformed. We have
now investigated whether PGD2/E2-like isoprostanes are also produced by rearrangement
of the PGG2-like intermediates involved in isoprostane formation. Using a variety of …
compounds (F2-isoprostanes) are produced in vivo independent of the cyclooxygenase as
products of free radical-catalyzed lipid peroxidation. F2-isoprostanes are initially formed in
situ from arachidonic acid esterified to phospholipids and then released preformed. We have
now investigated whether PGD2/E2-like isoprostanes are also produced by rearrangement
of the PGG2-like intermediates involved in isoprostane formation. Using a variety of …