[HTML][HTML] Modulation of chloride secretion in the rat ileum by intracellular bicarbonate
Increasing intracellular bicarbonate concentration ([HCO3−] i) inhibits calcium-mediated Cl−
secretion in rat distal colon and T84 cells. We investigated the effect of [HCO3−] i on Cl−
secretion in rat ileum. Segments of intact ileum from Sprague–Dawley rats were studied in
Ussing chambers and villus and crypt intracellular pH and [HCO3−] i were determined using
BCECF. A range of crypt and villus [HCO3−] i from 0 to 31 mM was obtained by varying
Ringer's composition. Basal serosal-to-mucosal Cl− flux (JClsm) averaged 8.5±0.2 μeq· h …
secretion in rat distal colon and T84 cells. We investigated the effect of [HCO3−] i on Cl−
secretion in rat ileum. Segments of intact ileum from Sprague–Dawley rats were studied in
Ussing chambers and villus and crypt intracellular pH and [HCO3−] i were determined using
BCECF. A range of crypt and villus [HCO3−] i from 0 to 31 mM was obtained by varying
Ringer's composition. Basal serosal-to-mucosal Cl− flux (JClsm) averaged 8.5±0.2 μeq· h …