Regulation of microautophagy and basal protein turnover in rat liver. Effects of short-term starvation.
GE Mortimore, BR Lardeux, CE Adams - Journal of Biological Chemistry, 1988 - ASBMB
Basal rates of long-lived (resident) protein degradation in rat liver, measured during
perfusion after amino acid suppression of macroautophagy, were shown to be strongly
regulated by caloric deprivation, decreasing 70% over 48 h in animals fed a high protein diet
and 50% in normal controls. Intralysosomal pools of degradable protein correlated directly
with basal turnover over this range, yielding a slope (0.09 min-1) that was virtually identical
with previous estimates of macroautophagic turnover. The specific radioactivity of valine …
perfusion after amino acid suppression of macroautophagy, were shown to be strongly
regulated by caloric deprivation, decreasing 70% over 48 h in animals fed a high protein diet
and 50% in normal controls. Intralysosomal pools of degradable protein correlated directly
with basal turnover over this range, yielding a slope (0.09 min-1) that was virtually identical
with previous estimates of macroautophagic turnover. The specific radioactivity of valine …
