Characterization of the dermatan sulfate proteoglycans, DS-PGI and DS-PGII, from bovine articular cartilage and skin isolated by octyl-sepharose chromatography
HU Choi, TL Johnson, S Pal, LH Tang… - Journal of Biological …, 1989 - ASBMB
Two forms of dermatan sulfate proteoglycans, called DS-PGI and DS-PGII, have been
isolated from both bovine fetal skin and calf articular cartilage and characterized. The
proteoglycans were isolated using either (a) molecular sieve chromatography under
conditions where DS-PGI selectively self-associates or (b) chromatography on octyl-
Sepharose, which separates DS-PGI from DS-PGII based on differences in the hydrophobic
properties of their core proteins. The NH 2-terminal amino acid sequence of DS-PGI from …
isolated from both bovine fetal skin and calf articular cartilage and characterized. The
proteoglycans were isolated using either (a) molecular sieve chromatography under
conditions where DS-PGI selectively self-associates or (b) chromatography on octyl-
Sepharose, which separates DS-PGI from DS-PGII based on differences in the hydrophobic
properties of their core proteins. The NH 2-terminal amino acid sequence of DS-PGI from …
