Protein phosphatases are involved in many cellular processes. One of the most abundant of
these enzymes, the serine/threonine-specific protein phosphatase type 2A (PP2A), is
present in most eukaryotic cells and serves a variety of functions. However, the detailed
study of its regulation and function has been hampered by the difficulty of manipulating its
expression level in cell culture. By using a new mammalian expression vector to forcibly
overexpress PP2A in the mouse fibroblast cell line NIH3T3, we now show that the catalytic …