The fluorolysis assay, a highly sensitive method for measuring the cytolytic activity of T cells at very low numbers
N Kienzle, S Olver, K Buttigieg, A Kelso - Journal of immunological …, 2002 - Elsevier
We have developed a highly sensitive cytolysis test, the fluorolysis assay, as a simple
nonradioactive and inexpensive alternative to the standard 51Cr-release assay. P815 cells
were stably transfected with a plasmid expressing the enhanced green fluorescent protein
(EGFP) gene. These target cells were coated with or without cognate peptide or anti-CD3 Ab
and then incubated with CD8+ T cells to allow antigen-specific or nonspecific lysis. The
degree of target cell lysis was measured using flow cytometry to count the percentage of …
nonradioactive and inexpensive alternative to the standard 51Cr-release assay. P815 cells
were stably transfected with a plasmid expressing the enhanced green fluorescent protein
(EGFP) gene. These target cells were coated with or without cognate peptide or anti-CD3 Ab
and then incubated with CD8+ T cells to allow antigen-specific or nonspecific lysis. The
degree of target cell lysis was measured using flow cytometry to count the percentage of …
