Repression of pentose phosphate pathway dehydrogenase synthesis and mRNA by dietary fat in rats
JE Tomlinson, R Nakayama, D Holten - The Journal of nutrition, 1988 - Elsevier
We have studied the effects of polyunsaturated fatty acid and its metabolism on the activity,
relative synthesis and mRNA levels for rat hepatic glucose-6-phosphate dehydrogenase
(G6PD) and 6-phosphogluconate dehydrogenase (6PGD). Rats were meal-fed high
carbohydrate diets containing either no fat, 5% safflower oil or 5% safflower oil+ eicosa-5, 8,
11, 14-tetraynoic acid (TYA). Hepatocytes were isolated and used as a source of RNA, de
novo radiolabeled protein and postmitochondrial supernatant for enzyme assay. Dietary …
relative synthesis and mRNA levels for rat hepatic glucose-6-phosphate dehydrogenase
(G6PD) and 6-phosphogluconate dehydrogenase (6PGD). Rats were meal-fed high
carbohydrate diets containing either no fat, 5% safflower oil or 5% safflower oil+ eicosa-5, 8,
11, 14-tetraynoic acid (TYA). Hepatocytes were isolated and used as a source of RNA, de
novo radiolabeled protein and postmitochondrial supernatant for enzyme assay. Dietary …
