[HTML][HTML] Inactivation of caspase-8 on mitochondria of Bcl-xL-expressing MCF7-Fas cells: role for the bifunctional apoptosis regulator protein
AH Stegh, BC Barnhart, J Volkland… - Journal of Biological …, 2002 - ASBMB
Apoptosis induction through CD95 (APO-1/Fas) critically depends on generation of active
caspase-8 at the death-inducing signaling complex (DISC). Depending on the cell type,
active caspase-8 either directly activates caspase-3 (type I cells) or relies on mitochondrial
signal amplification (type II cells). In MCF7-Fas cells that are deficient for pro-caspase-3,
even high amounts of caspase-8 produced at the DISC cannot directly activate downstream
effector caspases without mitochondrial help. Overexpression of Bcl-x L in these cells …
caspase-8 at the death-inducing signaling complex (DISC). Depending on the cell type,
active caspase-8 either directly activates caspase-3 (type I cells) or relies on mitochondrial
signal amplification (type II cells). In MCF7-Fas cells that are deficient for pro-caspase-3,
even high amounts of caspase-8 produced at the DISC cannot directly activate downstream
effector caspases without mitochondrial help. Overexpression of Bcl-x L in these cells …
