Eukaryotic protein synthesis initiation factor 2 (eIF‐2) can be phosphorylated on its α subunit by two well‐characterised protein kinases, termed the haem‐controlled repressor (HCR) and the double‐stranded RNA‐activated inhibitor (dsl). Phosphorylation of eIF‐2 by these kinases is thought to be important in the regulation of peptide‐chain initiation. We report the location of the serine residue in the α subunit, which is phosphorylated by both these enzymes. Limited tryptic digestion and subsequent cyanogen bromide treatment of rat liver eIF‐2 phosphorylated by HCR yielded one major phosphopeptide. This peptide had the sequence The same major phosphopeptide was obtained from rabbit reticulocyte eIF‐2 phosphorylated by HCR or dsI as judged by its behaviour on two‐dimensional mapping and reverse‐phase chromatography. In all cases the phosphorylated residue was found to be serine‐7, and not serine‐4, of the above sequence as determined from sequence analysis and by subdigestion of the peptide with Staphylococcus aureus V8 proteinase.