A study on CD45 isoform expression during T-cell development and selection events in the human thymus
K Fukuhara, M Okumura, H Shiono, M Inoue… - Human immunology, 2002 - Elsevier
CD45 molecules are known to appear as various isoforms generated by alternative splicing
of variable exons 4, 5, and 6, but the detailed profile of CD45 isoform expression during
thymocyte development has not been revealed. We examined the CD45 isoforms expressed
in the various human thymocytes' subsets defined by CD3, CD4, and CD8 expressions
using RT-PCR and 4-color flow cytometry. RT-PCR study revealed that RABC, RAB, RBC,
RB, and R0 isoforms were expressed in thymocytes while any of RAC, RA, or RC isoforms …
of variable exons 4, 5, and 6, but the detailed profile of CD45 isoform expression during
thymocyte development has not been revealed. We examined the CD45 isoforms expressed
in the various human thymocytes' subsets defined by CD3, CD4, and CD8 expressions
using RT-PCR and 4-color flow cytometry. RT-PCR study revealed that RABC, RAB, RBC,
RB, and R0 isoforms were expressed in thymocytes while any of RAC, RA, or RC isoforms …