Side-chain cleavage of 4-cholesten-3-one, 5-cholesten-3α-ol, β-sitosterol, and related steroids in endocrine tissues from rat and man
L Aringer, P Eneroth, L Nordström - Journal of Steroid Biochemistry, 1979 - Elsevier
Abstract Fourteen 4-14 C-labeled C 27-and C 29-steroids were incubated with mitochondria
prepared from rat adrenals, ovaries, testes and placenta, fortified with NADPH. Of the eleven
C 27-steroids six were available also with 26-14 C-label. The main purpose was to study
which of the substrates tested could be converted to C 21 and/or C 19-steroids. When
experiments with a 4-14 C-and a 26-14 C-labeled steroid were run in parallel, the
metabolites not labeled in 26-14 C-experiments but in 4-14 C-experiments were considered …
prepared from rat adrenals, ovaries, testes and placenta, fortified with NADPH. Of the eleven
C 27-steroids six were available also with 26-14 C-label. The main purpose was to study
which of the substrates tested could be converted to C 21 and/or C 19-steroids. When
experiments with a 4-14 C-and a 26-14 C-labeled steroid were run in parallel, the
metabolites not labeled in 26-14 C-experiments but in 4-14 C-experiments were considered …
