Radioactive labeling of antibody: a simple and efficient method
DJ Hnatowich, WW Layne, RL Childs, D Lanteigne… - Science, 1983 - science.org
DJ Hnatowich, WW Layne, RL Childs, D Lanteigne, MA Davis, TW Griffin, PW Doherty
Science, 1983•science.orgA simple and efficient method of covalently coupling the strong chelator
diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling
immunoglobulin G antibodies. After being coupled and labeled with indium-111, a
monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in
vitro and in vivo. In nude mice with a human colorectal xenograft, 41 percent of the injected
radioactivity became localized in each gram of xenograft at 24 hours compared with 9 …
diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling
immunoglobulin G antibodies. After being coupled and labeled with indium-111, a
monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in
vitro and in vivo. In nude mice with a human colorectal xenograft, 41 percent of the injected
radioactivity became localized in each gram of xenograft at 24 hours compared with 9 …
A simple and efficient method of covalently coupling the strong chelator diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling immunoglobulin G antibodies. After being coupled and labeled with indium-111, a monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in vitro and in vivo. In nude mice with a human colorectal xenograft, 41 percent of the injected radioactivity became localized in each gram of xenograft at 24 hours compared with 9 percent for control antibody and 19 percent for radioiodinated antibody to carcinoembryonic antigen.
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