Highly purified scavenger receptor class B, type I reconstituted into phosphatidylcholine/cholesterol liposomes mediates high affinity high density lipoprotein binding …
B Liu, M Krieger - Journal of Biological Chemistry, 2002 - ASBMB
The murine class B, type I scavenger receptor mSR-BI is a high and low density lipoprotein
(HDL and LDL) receptor that mediates selective uptake of cholesteryl esters. Here we
describe a reconstituted phospholipid/cholesterol liposome assay of the binding and
selective uptake activities of SR-BI derived from detergent-solubilized cells. The assay,
employing lysates from epitope-tagged receptor (mSR-BI-t1)-expressing mammalian and
insect cells, recapitulated many features of SR-BI activity in intact cells, including high affinity …
(HDL and LDL) receptor that mediates selective uptake of cholesteryl esters. Here we
describe a reconstituted phospholipid/cholesterol liposome assay of the binding and
selective uptake activities of SR-BI derived from detergent-solubilized cells. The assay,
employing lysates from epitope-tagged receptor (mSR-BI-t1)-expressing mammalian and
insect cells, recapitulated many features of SR-BI activity in intact cells, including high affinity …
