[HTML][HTML] Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins
Three sterol regulatory element-binding proteins (SREBP-1a,-1c, and-2) stimulate
transcription of genes involved in synthesis and receptor-mediated uptake of cholesterol and
fatty acids. Here, we explore the individual roles of each SREBP by preparing lines of
Chinese hamster ovary (CHO) cells that express graded amounts of nuclear forms of each
SREBP (designated nSREBPs) under control of a muristerone-inducible nuclear receptor
system. The parental hamster cell line (M19 cells) lacks its own nSREBPs, owing to a …
transcription of genes involved in synthesis and receptor-mediated uptake of cholesterol and
fatty acids. Here, we explore the individual roles of each SREBP by preparing lines of
Chinese hamster ovary (CHO) cells that express graded amounts of nuclear forms of each
SREBP (designated nSREBPs) under control of a muristerone-inducible nuclear receptor
system. The parental hamster cell line (M19 cells) lacks its own nSREBPs, owing to a …