Vascular endothelial growth factor-driven glioma growth and vascularization in an orthotopic rat model monitored by magnetic resonance imaging
RH Goldbrunner, M Bendszus, M Sasaki… - …, 2000 - journals.lww.com
OBJECTIVE: The goal of the present study was to develop an orthotopic in vivo model for the
investigation of vascular endothelial growth factor (VEGF)-dependent glioma growth and
vascularization. METHODS: C6 glioma cells were infected with viruses encoding sense or
antisense VEGF. Expression of the transgene was controlled by Northern blot analysis,
Western blot analysis, and immunohistochemistry. Spheroids generated from both clones as
well as from wild-type and mock-transfected cells were implanted in the brains of Sprague …
investigation of vascular endothelial growth factor (VEGF)-dependent glioma growth and
vascularization. METHODS: C6 glioma cells were infected with viruses encoding sense or
antisense VEGF. Expression of the transgene was controlled by Northern blot analysis,
Western blot analysis, and immunohistochemistry. Spheroids generated from both clones as
well as from wild-type and mock-transfected cells were implanted in the brains of Sprague …
