Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.
S Cotecchia, DA Schwinn… - Proceedings of the …, 1988 - National Acad Sciences
Proceedings of the National Academy of Sciences, 1988•National Acad Sciences
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with
oligonucleotides corresponding to the partial amino acid sequence of the receptor protein
purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes
a 515-residue polypeptide that shows the most sequence identity with the other adrenergic
receptors and the putative protein product of the related clone G-21. Similarities with the
muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm …
oligonucleotides corresponding to the partial amino acid sequence of the receptor protein
purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes
a 515-residue polypeptide that shows the most sequence identity with the other adrenergic
receptors and the putative protein product of the related clone G-21. Similarities with the
muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm …
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.
National Acad Sciences
