Adenovirus-mediated Transfer of a Wild-Type p53 Gene and Induction of Apoptosis in Cervical Cancer
K Hamada, R Alemany, WW Zhang, WN Hittelman… - Cancer research, 1996 - AACR
K Hamada, R Alemany, WW Zhang, WN Hittelman, R Lotan, JA Roth, MF Mitchell
Cancer research, 1996•AACRIn most cervical cancers, the function of p53 is down regulated. To explore the potential use
of p53 in gene therapy for cervical cancer, we introduced wild-type p53 into cervical cancer
cell lines via a recombinant adenoviral vector, Ad5CMV-p53, and analyzed its effects on cell
and tumor growth. The transduction efficiencies of all cell lines were 100% at a multiplicity of
infection of 100 or greater. The p53 protein was detected in Ad5CMV-p53-infected cells.
Protein expression peaked at day 3 after infection and lasted 15 days. The Ad5CMV-p53 …
of p53 in gene therapy for cervical cancer, we introduced wild-type p53 into cervical cancer
cell lines via a recombinant adenoviral vector, Ad5CMV-p53, and analyzed its effects on cell
and tumor growth. The transduction efficiencies of all cell lines were 100% at a multiplicity of
infection of 100 or greater. The p53 protein was detected in Ad5CMV-p53-infected cells.
Protein expression peaked at day 3 after infection and lasted 15 days. The Ad5CMV-p53 …
Abstract
In most cervical cancers, the function of p53 is down regulated. To explore the potential use of p53 in gene therapy for cervical cancer, we introduced wild-type p53 into cervical cancer cell lines via a recombinant adenoviral vector, Ad5CMV-p53, and analyzed its effects on cell and tumor growth. The transduction efficiencies of all cell lines were 100% at a multiplicity of infection of 100 or greater. The p53 protein was detected in Ad5CMV-p53-infected cells. Protein expression peaked at day 3 after infection and lasted 15 days. The Ad5CMV-p53-infected cells underwent apoptosis, and cell growth was greatly suppressed. The Ad5CMV-p53 treatment significantly reduced the volumes of established s.c. tumors in vivo. These results indicate that transfection of cervical cancer cells with the wild-type p53 gene via Ad5CMV-p53 is a potential novel approach to the therapy of cervical cancer.
AACR
